Life sciences

Automated Segmentation of Intracellular Substructures in Electron Microscopy (ASEM) on AWS

The Automated Segmentation of intracellular substructures in Electron Microscopy (ASEM) project provides deep learning models trained to segment structures in 3D images of cells acquired by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). Each model is trained to detect a single type of structure (mitochondria, endoplasmic reticulum, golgi apparatus, nuclear pores, clathrin-coated pits) in cells prepared via chemically-fixation (CF) or high-pressure freezing and freeze substitution (HPFS). You can use our open source pipeline to load a model and predict a class of sub-cellular structures in naive FIB-SEM cells images. If...

Listed for discovery. Access is provided by the original publisher under its terms.

Data coverage

The Automated Segmentation of intracellular substructures in Electron Microscopy (ASEM) project provides deep learning models trained to segment structures in 3D images of cells acquired by Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). Each model is trained to detect a single type of structure (mitochondria, endoplasmic reticulum, golgi apparatus, nuclear pores, clathrin-coated pits) in cells prepared via chemically-fixation (CF) or high-pressure freezing and freeze substitution (HPFS). You can use our open source pipeline to load a model and predict a class of sub-cellular structures in naive FIB-SEM cells images. If required, a fine-tuning procedure allows a model to be trained on a small amount of additional ground truth annotations to improve a prediction on a naive dataset. Together with the trained models, we also provide the training, validation and test datasets.

Access & permitted use

Review the publisher’s documentation for availability, eligibility and permitted uses, including commercial analysis, model training and redistribution.

All available datasets and models are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License

Need help sourcing data?

Tell us your required fields, population, geography and time period. We can review suitable sources and licensing options.

Request sourcing help

Related data sources