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Genomics

The Singapore Nanopore Expression Data Set

The Singapore Nanopore Expression (SG-NEx) project is an international collaboration to generate reference transcriptomes and a comprehensive benchmark data set for long read RNA-Seq. Transcriptome profiling is done using Nanopore PCR-cDNA sequencing (PCR-cDNA), Nanopore amplification-free cDNA sequencing (direct cDNA), Nanopore direct sequencing of native RNA (direct RNA), PacBio IsoSeq, and short read RNA-Seq. The SG-NEx core data includes 5 of the most commonly used cell lines and it is extended with additional cell lines and samples that cover a broad range of human tissues. All core samples are sequenced with at least 3...

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Data coverage

The Singapore Nanopore Expression (SG-NEx) project is an international collaboration to generate reference transcriptomes and a comprehensive benchmark data set for long read RNA-Seq. Transcriptome profiling is done using Nanopore PCR-cDNA sequencing (PCR-cDNA), Nanopore amplification-free cDNA sequencing (direct cDNA), Nanopore direct sequencing of native RNA (direct RNA), PacBio IsoSeq, and short read RNA-Seq. The SG-NEx core data includes 5 of the most commonly used cell lines and it is extended with additional cell lines and samples that cover a broad range of human tissues. All core samples are sequenced with at least 3 high quality replicates. For a subset of samples spike-in RNAs are used and matched m6A profiling data is available.

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CC BY-NC 4.0

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